A Flexible System of Enzymatic Analysis by Oliver H. Lowry

By Oliver H. Lowry

Show description

Read Online or Download A Flexible System of Enzymatic Analysis PDF

Similar botany books

Agrobacterium Protocols

Agrobacterium tumefaciens is a soil bacterium that for greater than a century has been referred to as a pathogen inflicting the plant crown gall illness. not like many different pathogens, Agrobacterium has the power to bring DNA to plant cells and completely adjust the plant genome. the invention of this targeted function 30 years in the past has supplied plant scientists with a robust device to genetically remodel vegetation for either easy study reasons and for agric- tural improvement.

Structural Wood Detailing in CAD Format

Structural wooden Detailing in CAD structure is a reference library for wooden construction development. Engineers, architects, and contractors will locate the e-book precious for day-by-day use and perform. It presents structural information for development, that's a significant component in construction layout. utilizing a library of normal info saves a lot of the engineering time and cash which was once spent drawing an identical information again and again.

Additional info for A Flexible System of Enzymatic Analysis

Example text

Under 5 μΐ the colorimetric method is required. Calibration of Pipettes 55 Although is it desirable to have available accurately calibrated pipettes, in most analyses this is not essential, since the pipette volumes will cancel out of the final calculation if standard solutions and samples are handled with the same pipettes. The calibration of pipettes is one of the best ways to test pipetting technique. Failure to obtain satisfactory checks means faulty technique or bad pipettes. Since the gravimetric method is the most precise, it offers the most critical test, but only for larger pipettes.

For enzymes with Km9s of 50, 100, 200, and 500 μΜ, the minimal enzyme requirement for [S]0 = 100 μΜ would be 30, 50, 90, and 210 U/liter, respectively, and only 10 U/liter less if [S]0 = 1 μΜ. 2. A Kinetics Primer for the Tissue Analyst 32 When Ku is substantially larger than [S] 0 , Eq. (2-27) reduces to Vm = 4 KJt. Thus, if the KM is 200 μΜ, to complete the reaction (98%) in 20 min would require enzyme activity equal to 40 μΜ/min or 40 units per liter. One-Step Reactions with Two Substrates The majority of analytical reactions to be considered will involve two substrates (A, B).

Colorimetric calibration is also more likely to reveal bad technique since it can be used with the smallest pipettes, for which pipetting faults can cause very serious errors. GRAVIMETRIC CALIBRATION Constriction pipettes are calibrated by delivery of water into a vessel already containing a little water. The increase in weight of the vessel is measured on an analytical balance. A narrow-mouthed vial without stopper and with a capacity not more than 20 times the volume of the pipette is recommended.

Download PDF sample

Rated 4.06 of 5 – based on 35 votes